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Assessment of Population and ECM Production Using Multiphoton Microscopy as an Indicator of Cell Viability

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Cell Viability Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1601))

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Abstract

Multiphoton microscopy allows continuous depth-resolved, nondestructive imaging of scaffold-seeded cells during cell or tissue culture. Spectrally separated images in high resolution can be provided while cells are conserved in their native state. Here we describe the seeding of mesenchymal stem cells to bacterial nanocellulose hydropolymer scaffolds followed by 2-channel imaging of cellular autofluorescence (AF) and collagen-I formation using second harmonic generation (SHG) signals. With this approach the simultaneous observation of the progression of cell morphology and production of extracellular matrix as hallmarks of viability and cell fitness is possible.

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Acknowledgments

This work was supported by the Emerging Fields Initiative (EFI) of the University of Erlangen-Nürnberg (project TOPbiomat) and the Erlangen Graduate School in Advanced Optical Technologies (SAOT) within the German Excellence Initiative. We want to thank Dana Kralisch and Nadine Hessler (JeNaCell GmbH) for providing BNC fleeces.

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Authors and Affiliations

  1. Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Paul-Gordan-Street 3, Erlangen, 91052, Germany

    Martin Vielreicher & Oliver Friedrich

Authors
  1. Martin Vielreicher
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  2. Oliver Friedrich
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Corresponding author

Correspondence to Martin Vielreicher .

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Editors and Affiliations

  1. Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany

    Daniel F. Gilbert

  2. Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany

    Oliver Friedrich

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Vielreicher, M., Friedrich, O. (2017). Assessment of Population and ECM Production Using Multiphoton Microscopy as an Indicator of Cell Viability. In: Gilbert, D., Friedrich, O. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 1601. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6960-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6960-9_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6959-3

  • Online ISBN: 978-1-4939-6960-9

  • eBook Packages: Springer Protocols

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