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ATM Kinase pp 57–70Cite as

ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using Cellomics™ ArrayScan VTI Platform

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1599))

Abstract

Small molecule inhibitors of the ATM pathway could represent a promising opportunity for cancer therapy, working either by enhancing the clinical efficacy of radiotherapy and existing chemotherapies or by synthetic lethality-based mechanisms. In this chapter, we describe a high-throughput, high-content imaging assay monitoring levels of ATM phosphorylation at Serine 1981 following induction of DNA damage by ionizing radiation.

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Author information

Authors and Affiliations

  1. Discovery Sciences iMed, AstraZeneca, Global HTS Centre, Mereside, Alderley Park, Macclesfield, SK10 4TG, Cheshire, UK

    Catherine Bardelle

  2. Lead Discovery Center GmbH, Otto-Hahn-Str. 15, D-44227, Dortmund, Germany

    Joanna Boros

Authors
  1. Catherine Bardelle
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  2. Joanna Boros
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Corresponding author

Correspondence to Joanna Boros .

Editor information

Editors and Affiliations

  1. University of Queensland Centre for Clinical Research (UQCCR), University of Queensland, Herston, Queensland, Australia

    Sergei V. Kozlov

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Bardelle, C., Boros, J. (2017). ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using Cellomics™ ArrayScan VTI Platform. In: Kozlov, S. (eds) ATM Kinase. Methods in Molecular Biology, vol 1599. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6955-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6955-5_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6953-1

  • Online ISBN: 978-1-4939-6955-5

  • eBook Packages: Springer Protocols

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