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A Proteomic Evaluation of Sympathetic Activity Biomarkers of the Hypothalamus-Pituitary-Adrenal Axis by Western Blotting Technique Following Experimental Traumatic Brain Injury

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Neuroproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1598))

Abstract

Endocrine disorders and autonomic dysfunction are common paradigms following traumatic brain injury (TBI). Alterations in the hypothalamus–pituitary–adrenal (HPA) axis following TBI may result in impaired vasopressor response, energy imbalance, fatigue, depression, or neurological disorders. Autonomic dysfunction is a common disorder following TBI. The sympathetic activity markers on HPA axis can be measured by Western blot protein analysis. Tyrosine hydroxylase, dopamine beta hydroxylase are the key enzymes for the synthesis of norepinephrine; and neuropeptide Y (NPY) is the peptide that is co-stored and co-released with norepinephrine. Thus, the present chapter reviews the experimental protocols for Western blot protein analysis for the measurement of biomarkers that indicate sympathetic activity in brain regions (hypothalamus, pituitary, cerebral cortex, and cerebellum) following TBI.

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References

  1. CDC (2010) TBI data and statistics.

    Google Scholar 

  2. Kobeissy F, Mondello S, Tumer N, Toklu HZ, Whidden MA, Kirichenko N, Zhang Z, Prima V, Yassin W, Anagli J, Chandra N, Svetlov S, Wang KK (2013) Assessing neuro-systemic & behavioral components in the pathophysiology of blast-related brain injury. Front Neurol 4:186

    Article  PubMed  PubMed Central  Google Scholar 

  3. Toklu HZ, Tumer N (2015) Oxidative stress, brain edema, blood-brain barrier permeability, and autonomic dysfunction from traumatic brain injury. In: Kobeissy FH (ed) Brain neurotrauma: molecular, neuropsychological, and rehabilitation aspects. CRC Press/Taylor & Francis; Boca Raton, FL

    Google Scholar 

  4. Toklu HZ, Muller-Delp J, Yang Z, Oktay S, Sakarya Y, Strang K, Ghosh P, Delp MD, Scarpace PJ, Wang KK, Tumer N (2015) The functional and structural changes in the basilar artery due to overpressure blast injury. J Cereb Blood Flow Metab 35:1950–1956

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Cernak I, Noble-Haeusslein LJ (2010) Traumatic brain injury: an overview of pathobiology with emphasis on military populations (vol 30, pg 255, 2010). J Cereb Blood Flow Metab 30:1262–1262

    Article  PubMed Central  Google Scholar 

  6. McConeghy KW, Hatton J, Hughes L, Cook AM (2012) A review of neuroprotection pharmacology and therapies in patients with acute traumatic brain injury. CNS Drugs 26:613–636

    Article  CAS  PubMed  Google Scholar 

  7. Tran LV (2014) Understanding the pathophysiology of traumatic brain injury and the mechanisms of action of neuroprotective interventions. J Trauma Nurs 21:30–35

    Article  PubMed  Google Scholar 

  8. Hendricks HT, Heeren AH, Vos PE (2010) Dysautonomia after severe traumatic brain injury. Eur J Neurol 17:1172–1177

    Article  CAS  PubMed  Google Scholar 

  9. Lv LQ, Hou LJ, Yu MK, Qi XQ, Chen HR, Chen JX, Hu GH, Luo C, Lu YC (2011) Risk factors related to dysautonomia after severe traumatic brain injury. J Trauma 71:538–542

    PubMed  Google Scholar 

  10. Laxe S, Terre R, Leon D, Bernabeu M (2013) How does dysautonomia influence the outcome of traumatic brain injured patients admitted in a neurorehabilitation unit? Brain Inj 27:1383–1387

    Article  PubMed  Google Scholar 

  11. Viola-Saltzman M, Musleh C (2016) Traumatic brain injury-induced sleep disorders. Neuropsychiatr Dis Treat 12:339–348

    Article  PubMed  PubMed Central  Google Scholar 

  12. Kanjwal K, Karabin B, Kanjwal Y, Grubb BP (2010) Autonomic dysfunction presenting as postural orthostatic tachycardia syndrome in patients with multiple sclerosis. Int J Med Sci 7:62–67

    Article  PubMed  PubMed Central  Google Scholar 

  13. De Tanti A, Gasperini G, Rossini M (2005) Paroxysmal episodic hypothalamic instability with hypothermia after traumatic brain injury. Brain Inj 19:1277–1283

    Article  PubMed  Google Scholar 

  14. Cernak I (2010) The importance of systemic response in the pathobiology of blast-induced neurotrauma. Front Neurol 1:151

    Article  PubMed  PubMed Central  Google Scholar 

  15. Jacobson L (2005) Hypothalamic-pituitary-adrenocortical axis regulation. Endocrinol Metab Clin North Am 34:271–292. vii

    Article  CAS  PubMed  Google Scholar 

  16. Webb NE, Little B, Loupee-Wilson S, Power EM (2014) Traumatic brain injury and neuro-endocrine disruption: medical and psychosocial rehabilitation. NeuroRehabilitation 34:625–636

    PubMed  Google Scholar 

  17. Tanriverdi F, Schneider HJ, Aimaretti G, Masel BE, Casanueva FF, Kelestimur F (2015) Pituitary dysfunction after traumatic brain injury: a clinical and pathophysiological approach. Endocr Rev 36:305–342

    Article  CAS  PubMed  Google Scholar 

  18. Taylor AN, Rahman SU, Sanders NC, Tio DL, Prolo P, Sutton RL (2008) Injury severity differentially affects short- and long-term neuroendocrine outcomes of traumatic brain injury. J Neurotrauma 25:311–323

    Article  PubMed  Google Scholar 

  19. McIntosh TK, Yu T, Gennarelli TA (1994) Alterations in regional brain catecholamine concentrations after experimental brain injury in the rat. J Neurochem 63:1426–1433

    Article  CAS  PubMed  Google Scholar 

  20. Tumer N, Svetlov S, Whidden M, Kirichenko N, Prima V, Erdos B, Sherman A, Kobeissy F, Yezierski R, Scarpace PJ, Vierck C, Wang KK (2013) Overpressure blast-wave induced brain injury elevates oxidative stress in the hypothalamus and catecholamine biosynthesis in the rat adrenal medulla. Neurosci Lett 544:62–67

    Article  CAS  PubMed  Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  22. Toklu HZ, Kwon OS, Sakarya Y, Powers SK, Llinas K, Kirichenko N, Sollanek KJ, Wiggs MP, Smuder AJ, Talbert EE, Scarpace PJ, Tumer N (2014) The effects of enalapril and losartan on mechanical ventilation-induced sympathoadrenal activation and oxidative stress in rats. J Surg Res 188:510–516

    Article  CAS  PubMed  Google Scholar 

  23. Toklu HZ, Sakarya Y, Kirichenko N, Llinas K, Yang Z, Scarpace PJ, Wang KKW, Tümer N (2014) The autonomic dysfunction in different brain regions due to overpressure blast injury in rats. University of Florida Health Cancer Center Research Day, poster no: 70

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, University of Florida College of Medicine, ARB R5-234, 1200 Newell Drive, PO 100267, Gainesville, FL, 32610, USA

    Hale Zerrin Toklu, Yasemin Sakarya & Nihal Tümer

  2. Geriatric Research Education and Clinical Center, Malcolm Randall Veterans Affairs Medical Center, Gainesville, FL, USA

    Hale Zerrin Toklu & Nihal Tümer

  3. North Florida Regional Medical Center, Department of Graduate Medical Education, 6500 W Newberry Rd, Gainesville, 32605, FL, USA

    Hale Zerrin Toklu

Authors
  1. Hale Zerrin Toklu
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  2. Yasemin Sakarya
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  3. Nihal Tümer
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Corresponding author

Correspondence to Hale Zerrin Toklu .

Editor information

Editors and Affiliations

  1. Department of Psychiatry, University of Florida McKnight Brain Institute, Gainesville, Florida, USA

    Firas H. Kobeissy

  2. Department of Cell Biology, Microbiology, & Molecular Biology, University of South Florida, Tampa, Florida, USA

    Stanley M. Stevens, Jr.

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Toklu, H.Z., Sakarya, Y., Tümer, N. (2017). A Proteomic Evaluation of Sympathetic Activity Biomarkers of the Hypothalamus-Pituitary-Adrenal Axis by Western Blotting Technique Following Experimental Traumatic Brain Injury. In: Kobeissy, F., Stevens, Jr., S. (eds) Neuroproteomics. Methods in Molecular Biology, vol 1598. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6952-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6952-4_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6950-0

  • Online ISBN: 978-1-4939-6952-4

  • eBook Packages: Springer Protocols

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