Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells

Part of the Methods in Molecular Biology book series (MIMB, volume 1597)


Hair follicles develop from the ectoderm in embryos and cyclically regenerate using proper spatiotemporal signaling molecules, which are conserved in organogenesis during adulthood. Previously, we demonstrated that bioengineered hair follicle germs could regenerate functional hair follicles via a three-dimensional cell manipulation technique, which we named the “organ germ method.” We could also regulate the type of hair follicle and pigmentation with correct structures by rearranging the source of the cells. In this article, we describe a detailed protocol for the regeneration of functional hair follicles and their stem cell niches by the rearrangement of embryonic or adult hair follicle-derived epithelial and mesenchymal cells.

Key words

Organ germ method Bioengineered hair follicle Regeneration Organ culture Transplantation 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Kyosuke Asakawa
    • 1
  • Koh-ei Toyoshima
    • 1
    • 2
  • Takashi Tsuji
    • 1
    • 2
  1. 1.Laboratory for Organ RegenerationRIKEN Center for Developmental BiologyKobeJapan
  2. 2.Organ Technologies Inc.Minato-kuJapan

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