Functional Pituitary Tissue Formation

  • Chikafumi Ozone
  • Hidetaka Suga
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)


The adenohypophysis, which mainly consists of anterior pituitary, plays important roles for endocrine systems by secreting several hormones indispensable for maintaining homeostasis. During early mouse development, the pituitary primordium (called Rathke’s pouch) develops from oral ectoderm adjacent to ventral hypothalamus by interaction between these two tissues. By using mouse embryonic stem cells (ESCs), we recapitulated this in vivo micro-environment of the pituitary development and demonstrated that Rathke’s pouch-like structures were self-formed from three-dimensional (3D) floating culture. The mouse ESC-derived Rathke’s pouch-like structures subsequently differentiated into hormone-producing cells such as corticotrophs and somatotrophs. We have modified this technique for human pluripotent stem cells and recently reported that pituitary placodes can also be generated from human ESCs through a similar process. Here, we describe a protocol for human ESC culture for in vitro generation of 3D pituitary tissue.

Key words

Pituitary gland Hypothalamus Embryonic stem cell Pluripotent stem cell Development Regenerative medicine Self-organization 3D culture Aggregate 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Endocrinology and Diabetes, Graduate School of MedicineNagoya UniversityNagoyaJapan
  2. 2.Laboratory for Organ RegenerationRIKEN Center for Developmental BiologyKobeJapan
  3. 3.Department of Endocrinology and DiabetesNagoya University HospitalNagoyaJapan

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