Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)

Abstract

A three-dimensional (3D) tissue generated in vitro is a promising source to study developmental biology and regenerative medicine. In the last decade, Yoshiki Sasai’s group have developed a 3D stem cell culture technique known as SFEBq and demonstrated that embryonic stem cells (ESCs) have an ability to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs.

Key words

SFEBq culture Human ESCs Retina Neural retina RPE Optic cup Ciliary margin 

Notes

Acknowledgments

This article is dedicated to late Dr. Yoshiki Sasai, a great mentor and gifted scientist who pioneered this field. A.K. is grateful to Drs. Koichi Saito, Toru Kimura, and the members of Sasai Lab and Eiraku Lab for fruitful discussion.

Competing financial interests

A.K. is employed by Sumitomo Dainippon Pharma Co., Ltd. T.N. is employed by Sumitomo Chemical Co., Ltd. The authors are inventors on patent applications.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.In Vitro Histogenesis teamRIKEN Center for Developmental BiologyKobeJapan
  2. 2.Regenerative and Cellular Medicine OfficeSumitomo Dainippon Pharma Co., Ltd.Chuo KobeJapan
  3. 3.Environmental Health Science LaboratorySumitomo Chemical Co., Ltd.OsakaJapan

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