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In Vivo Model of Small Intestine

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Organ Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1597))

Abstract

The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6949-4_18

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6949-4_18

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Author information

Authors and Affiliations

  1. Department of Pediatric General and Thoracic Surgery, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA

    Maxime M. Mahe, Nicole E. Brown, Holly M. Poling & Michael A. Helmrath

  2. Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA

    Maxime M. Mahe & Michael A. Helmrath

Authors
  1. Maxime M. Mahe
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  2. Nicole E. Brown
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  3. Holly M. Poling
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  4. Michael A. Helmrath
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Corresponding author

Correspondence to Michael A. Helmrath .

Editor information

Editors and Affiliations

  1. Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

    Takashi Tsuji

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Mahe, M.M., Brown, N.E., Poling, H.M., Helmrath, M.A. (2017). In Vivo Model of Small Intestine. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6949-4_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6947-0

  • Online ISBN: 978-1-4939-6949-4

  • eBook Packages: Springer Protocols

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