In Vivo Model of Small Intestine

  • Maxime M. Mahe
  • Nicole E. Brown
  • Holly M. Poling
  • Michael A. Helmrath
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)


The utilization of human pluripotent stem cells (hPSCs) offers new avenues in the generation of organs and opportunities to understand development and diseases. The hPSC-derived human intestinal organoids (HIOs) provide a new tool to gain insights in small intestinal development, physiology, and associated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

Key words

Bioengineered small intestine Human intestinal organoids Pluripotent stem cells Transplantation In vivo model Mesentery Kidney capsule 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Maxime M. Mahe
    • 1
    • 2
  • Nicole E. Brown
    • 1
  • Holly M. Poling
    • 1
  • Michael A. Helmrath
    • 1
    • 2
  1. 1.Department of Pediatric General and Thoracic SurgeryCincinnati Children’s Hospital Medical CenterCincinnatiUSA
  2. 2.Department of PediatricsUniversity of CincinnatiCincinnatiUSA

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