Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells

  • Taka-aki K. Noguchi
  • Akira Kurisaki
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)


In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.

Key words

Stomach organoids Embryonic stem cells Tissue formation Disease model Differentiation Shh Dkk-1 Barx1 


  1. 1.
    Schiesser JV A, Wells JM (2014) Generation of β cells from human pluripotent stem cells: are we there yet? Ann N Y Acad Sci 1311:124–137CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Gerbal-Chaloin S, Funakoshi N, Caillaud A et al (2014) Human induced pluripotent stem cells in hepatology: beyond the proof of concept. Am J Pathol 184:332–347CrossRefPubMedGoogle Scholar
  3. 3.
    Ghaedi M, Niklason LE, Williams J (2015) Development of lung epithelium from induced pluripotent stem cells. Curr Transplant Rep 2:81–89CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Kurmann AA, Serra M, Hawkins F et al (2015) Regeneration of thyroid function by transplantation of differentiated pluripotent stem cells. Cell Stem Cell 17:527–542CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    McCracken KW, Cata EM, Crawford CM et al (2014) Modeling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature 516:400–404CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Kim BM, Buchner G, Miletich I et al (2006) The stomach mesenchymal transcription factor Barx1 specifies gastric epithelial identity through inhibition of transient Wnt signaling. Dev Cell 8:611–622CrossRefGoogle Scholar
  7. 7.
    Jayewickreme CD, Shivdasani RA (2015) Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol 405:21–32CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Noguchi TK, Ninomiya N, Sekine M et al (2015) Generation of stomach tissue from mouse embryonic stem cells. Nat Cell Biol 17:984–993CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental SciencesThe University of TsukubaIbarakiJapan
  2. 2.Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan

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