Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)

Abstract

In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.

Key words

Stomach organoids Embryonic stem cells Tissue formation Disease model Differentiation Shh Dkk-1 Barx1 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental SciencesThe University of TsukubaIbarakiJapan
  2. 2.Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan

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