Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells
In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.
Key wordsStomach organoids Embryonic stem cells Tissue formation Disease model Differentiation Shh Dkk-1 Barx1