Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells

  • Yasuhiro Yoshimura
  • Atsuhiro Taguchi
  • Ryuichi Nishinakamura
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)

Abstract

The kidney is a vital organ that has an important role in the maintenance of homeostasis by fluid volume regulation and waste product excretion. This role cannot be performed without the three-dimensional (3D) structure of the kidney. Therefore, it is important to generate the 3D structure of the kidney when inducing functional kidney tissue or the whole organ from pluripotent stem cells. In this chapter, we describe the detailed methods to induce kidney progenitor cells from pluripotent stem cells, which are based on embryological development. We also provide a method to generate 3D kidney tissue with vascularized glomeruli upon transplantation.

Key words

Three-dimensional kidney structure Nephron progenitor cells Mouse embryonic stem cells Human-induced pluripotent stem cells Transplantation 

References

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Yasuhiro Yoshimura
    • 1
  • Atsuhiro Taguchi
    • 1
  • Ryuichi Nishinakamura
    • 1
  1. 1.Department of Kidney Development, Institute of Molecular Embryology and GeneticsKumamoto UniversityKumamotoJapan

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