Advertisement

Functional Salivary Gland Regeneration

  • Miho Ogawa
  • Takashi Tsuji
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1597)

Abstract

The salivary gland plays important roles in maintaining the health and homeostasis of the oral cavity by regulating functions, such as chewing, digestion, cleaning, and swallowing. Salivary gland dysfunction causes dry mouth syndrome, which includes many oral problems, such as dental caries, bacterial infection, mastication dysfunction, and swallowing dysfunction. Therefore, salivary gland regeneration is expected, and we attempted to establish a method by manual approaches. Previously, a novel three-dimensional cell manipulation technique, the “organ germ method,” to create a bioengineered organ germ enabled the regeneration of structurally correct and fully functional bioengineered organs, including the teeth, hair follicles, and secretory glands, in vivo. Here, we describe the protocol for salivary gland germ regeneration using this method and the functional analysis of the regenerated salivary gland.

Key words

Salivary gland Bioengineered salivary gland germ Organ germ method Saliva secretion Transplantation 

References

  1. 1.
    Avery JK (2002) Oral development and histology. Thieme, Berlin, pp 292–330Google Scholar
  2. 2.
    Knosp MW, Knox MS, Hoffman PM (2012) Salivary gland organogenesis. Wiley Interdiscip Rev Dev Biol 1:69–82CrossRefPubMedGoogle Scholar
  3. 3.
    Tucker AS, Miletich I (2010) Salivary glands; development, adaptations, and disease. Karger, LondonCrossRefGoogle Scholar
  4. 4.
    Jaskoll T, Melnick M (2004) Embryonic salivary gland branching morphogenesis. Madame Curie. Landes Bioscience, Austin (TX).Google Scholar
  5. 5.
    Edgar M, Dawes C, Mullane OD (2004) Saliva and oral health, 3rd edn. British Dental Association, LondonGoogle Scholar
  6. 6.
    Patel VN, Rebustini IT, Hoffman MP (2006) Salivary gland branching morphogenesis. Differentiation 74:349–364CrossRefPubMedGoogle Scholar
  7. 7.
    Sakai T, Larsen M, Yamada MK (2003) Fibronectin requirement in branching morphogenesis. Nature 423:876–881ACrossRefPubMedGoogle Scholar
  8. 8.
    Wei C, Larsen M, Hoffman MP, Yamada KM (2007) Self-organization and branching morphogenesis of primary salivary epithelial cells. Tissue Eng 13(4):721–735CrossRefPubMedGoogle Scholar
  9. 9.
    Kagami H, Wang S, Hai B (2008) Restoring the function of salivary glands. Oral Dis 14:15–24PubMedGoogle Scholar
  10. 10.
    Nakao K, Morita R, Saji Y et al (2007) The development of a bioengineered organ germ method. Nat Methods 4:227–230CrossRefPubMedGoogle Scholar
  11. 11.
    Ikeda E, Morita R, Nakao K et al (2009) Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci U S A 106:13475–13480CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Oshima M, Mizuno M, Imamura A et al (2011) Functional tooth regeneration using a bioengineered tooth unit as a mature organ replacement regenerative therapy. PLoS One 6:e21531CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Toyoshima K, Asakawa K, Ishibashi N et al (2012) Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches. Nat Commun 3:784CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Hirayama M, Ogawa M, Oshima M et al (2013) Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ. Nat Commun 4:2497PubMedPubMedCentralGoogle Scholar
  15. 15.
    Ogawa M, Oshima M, Imamura A et al (2013) Functional salivary gland regeneration by transplantation of a bioengineered organ germ. Nat Commun 4:2498CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Laboratory for Organ RegenerationRIKEN Center for Developmental BiologyKobeJapan
  2. 2.Organ Technologies Inc.Minato-kuJapan

Personalised recommendations