Abstract
Oxidation of lipids occurs in vivo with oxidative stress and during storage of biological samples, even at −80 °C. In this chapter, we demonstrate that the oxidation products of phosphatidylcholines include an aldehyde (sn-2 unsaturated fatty acyl substituent), an acid (sn-2 unsaturated fatty acyl substituent), the associated lysophosphatidylcholine, and a hydroperoxide (Fuchs et al., Lipids 42:991–998, 2007; Hui et al., Anal Bioanal Chem 403:1831–1840, 2012).
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References
Fuchs B, Müller K, Göritz F, Blottner S, Schiller J (2007) Characteristic oxidation products of choline plasmalogens are detectable in cattle and roe deer spermatozoa by MALDI-TOF mass spectrometry. Lipids 42:991–998
Hui SP, Taguchi Y, Takeda S, Ohkawa F, Sakurai T, Yamaki S, Jin S, Fuda H, Kurosawa T, Chiba H (2012) Quantitative determination of phosphatidylcholine hydroperoxides during copper oxidation of LDL and HDL by liquid chromatography/mass spectrometry. Anal Bioanal Chem 403:1831–1840
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This work was funded by Lincoln Memorial University.
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Wood, P. (2017). High-Resolution Mass Spectrometry of Glycerophospholipid Oxidation Products. In: Wood, P. (eds) Lipidomics. Neuromethods, vol 125. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6946-3_18
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DOI: https://doi.org/10.1007/978-1-4939-6946-3_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6946-3
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