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Peroxisomes pp 113–130Cite as

Detection and Immunolabeling of Peroxisomal Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1595))

Abstract

Peroxisomes are essential organelles in mammals which contribute to cellular lipid metabolism and redox homeostasis. The spectrum of their functions in human health and disease is far from being complete, and unexpected and novel roles of peroxisomes are being discovered. To date, those include novel biological roles in antiviral defence, as intracellular signaling platforms and as protective organelles in sensory cells. Furthermore, peroxisomes are part of a complex network of interacting subcellular compartments which involves metabolic cooperation, cross-talk and membrane contacts. As potentially novel peroxisomal proteins are continuously discovered, there is great interest in the verification of their peroxisomal localization. Here, we present protocols used successfully in our laboratory for the detection and immunolabeling of peroxisomal proteins in cultured mammalian cells. We present immunofluorescence and fluorescence-based techniques as well as reagents to determine peroxisome-specific targeting and localization of candidate proteins.

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Acknowledgments

We would like to thank A. Manner for providing images for Fig. 1d. This work was supported by the Marie Curie Initial Training Network (ITN) action (FP7-2012-PERFUME-316723) and the Biotechnology and Biological Sciences Research Council (BB/K006231/1; BB/N01541X/1).

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Authors and Affiliations

  1. College of Life and Environmental Sciences, Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, United Kingdom

    Tina A. Schrader & Michael Schrader

  2. Center for Biomedicine and Medical Technology Mannheim, Institute of Neuroanatomy, University of Heidelberg, Ludolf-Krehl Str. 13-17, 68137, Mannheim, Germany

    Markus Islinger

Authors
  1. Tina A. Schrader
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  2. Markus Islinger
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Correspondence to Michael Schrader .

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  1. College of Life and Environmental Sciences, Biosciences, University of Exeter, Exeter, United Kingdom

    Michael Schrader

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Schrader, T.A., Islinger, M., Schrader, M. (2017). Detection and Immunolabeling of Peroxisomal Proteins. In: Schrader, M. (eds) Peroxisomes. Methods in Molecular Biology, vol 1595. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6937-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6937-1_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6935-7

  • Online ISBN: 978-1-4939-6937-1

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