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Dynamic Measures of Flagellar Gene Expression

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Book cover The Bacterial Flagellum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1593))

Abstract

Many genes are required to assemble flagella. These genes encode not only the structural elements of the flagellum but also a number of regulators that control how the flagellar genes are temporally expressed during the assembly process. These regulators also specify the likelihood that a given cell will express the flagellar genes. In particular, not all cells express the flagellar genes, resulting in mixed populations of motile and non-motile cells. Nutrients provide one signal that specifies the motile fraction. In this chapter, we describe two methods for measuring flagellar gene expression dynamics using fluorescent proteins in Salmonella enterica. Both the methods can be used to investigate the mechanisms governing flagellar gene expression dynamics.

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Author information

Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave, Urbana, IL, 61801, USA

    Santosh Koirala & Christopher V. Rao

Authors
  1. Santosh Koirala
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  2. Christopher V. Rao
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Corresponding author

Correspondence to Christopher V. Rao .

Editor information

Editors and Affiliations

  1. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan

    Tohru Minamino

  2. Quantitative Biology Center RIKEN, Suita, Osaka, Japan

    Keiichi Namba

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Koirala, S., Rao, C.V. (2017). Dynamic Measures of Flagellar Gene Expression. In: Minamino, T., Namba, K. (eds) The Bacterial Flagellum. Methods in Molecular Biology, vol 1593. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6927-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6927-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6926-5

  • Online ISBN: 978-1-4939-6927-2

  • eBook Packages: Springer Protocols

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