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Food Allergens pp 79–94Cite as

Enzyme-Linked Immunosorbent Assay (ELISA)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1592))

Abstract

Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by “hidden”, “masked”, or “contaminated” proteins that are known major allergens. Many techniques have been developed to detect even small traces of food allergens, for clinical or laboratory purposes. Enzyme-linked immunosorbent assay (ELISA) is one of the best validated and most routinely used immunoassay in allergy research, in allergy diagnosis in allergy-related quality control in various industries. Although as a technique it has been implemented for the last 45 years, the evolution in biochemistry allowed the development of ultrasensitive ELISA variations that are capable of measuring quantities in the scale of picograms, rendering ELISA attractive, robust, and very famous.

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Author information

Authors and Affiliations

  1. Department of Allergy and Clinical Immunology, 424 General Military Training Hospital, 11 Eleftheriou Venizelou Street, Kalamaria, 55 133, Thessaloniki, Greece

    George N. Konstantinou

  2. Division of Allergy and Immunology, Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    George N. Konstantinou

Authors
  1. George N. Konstantinou
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Correspondence to George N. Konstantinou .

Editor information

Editors and Affiliations

  1. Bioinformatics Institute, A*STAR, Singapore, Singapore

    Jing Lin

  2. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, United Kingdom

    Marcos Alcocer

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Konstantinou, G.N. (2017). Enzyme-Linked Immunosorbent Assay (ELISA). In: Lin, J., Alcocer, M. (eds) Food Allergens. Methods in Molecular Biology, vol 1592. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6925-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6925-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6923-4

  • Online ISBN: 978-1-4939-6925-8

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