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T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens

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Food Allergens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1592))

Abstract

Characterization of allergen-specific T cells is critical to understand their contribution to disease pathogenesis. The identification of immunodominant T-cell epitopes is crucial for development of T-cell-based vaccines. Peptide-specific T-cell proliferation studies are usually performed in a library of short synthetic peptides (15mer or 20mer) with 3 or 5 offset spanning the entire length of the allergen. T-cell peptide epitopes lack the primary and tertiary structure of the native protein to cross-link IgE, but retain the ability to stimulate T cells. The peptides sequences can also be obtained either by in silico approaches and in vitro binding assays. The efficacy of T-cell epitope-based peptide immunotherapy has been proven in certain allergies. The present methodology describes T-cell proliferation assays using whole blood sample from allergic subjects.

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Author information

Authors and Affiliations

  1. Division of Allergy and Immunology, Department of Pediatrics, The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, Icahn Building, Room 11-23B, 1089, New York, NY, 10029, USA

    Madhan Masilamani

  2. Immunology Institute and The Mindich Child Health and Development Institute, Mount Sinai School of Medicine, New York, NY, 10029, USA

    Madhan Masilamani

  3. Immunology Department, CDB, Hospital Clinic de Barcelona, Universitat de Barcelona, Barcelona, Spain

    Mariona Pascal

  4. Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Hugh A. Sampson

  5. The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Hugh A. Sampson

Authors
  1. Madhan Masilamani
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  2. Mariona Pascal
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  3. Hugh A. Sampson
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Corresponding author

Correspondence to Madhan Masilamani .

Editor information

Editors and Affiliations

  1. Bioinformatics Institute, A*STAR, Singapore, Singapore

    Jing Lin

  2. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, United Kingdom

    Marcos Alcocer

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Masilamani, M., Pascal, M., Sampson, H.A. (2017). T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens. In: Lin, J., Alcocer, M. (eds) Food Allergens. Methods in Molecular Biology, vol 1592. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6925-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6925-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6923-4

  • Online ISBN: 978-1-4939-6925-8

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