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Stem Cell Banking pp 131–137Cite as

Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1590))

Abstract

Human somatic cells can be reprogrammed by defined factors to induced pluripotent stem cells (iPSCs). Importantly, the quality of iPSCs could impact the potential of these cells in basic and clinic research. Here, we describe a method to reprogram human fibroblast cells with Sendai virus in chemically defined conditions, to generate iPSCs that are integration-free and suitable for research and translational applications.

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References

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Acknowledgment

This work was supported by the NIH Center for Regenerative Medicine and the National Heart, Lung and Blood Institute, and G.C. is also supported by the University of Macau (MYRG2015-00228-FHS). Jeanette Beers helped to establish and edit the protocol.

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Authors and Affiliations

  1. Faculty of Health Sciences, University of Macau, Taipa, Macau, China

    Guokai Chen

  2. Center for Molecular Medicine, National Heart, Lung and Blood Institute, Bethesda, MD, USA

    Guokai Chen

  3. New York Stem Cell Foundation Research Institute, New York, NY, USA

    Mahendra Rao

  4. Q Therapeutics, Salt Lake City, UT, USA

    Mahendra Rao

  5. Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston-Salem, NC, USA

    Mahendra Rao

Authors
  1. Guokai Chen
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  2. Mahendra Rao
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Corresponding authors

Correspondence to Guokai Chen or Mahendra Rao .

Editor information

Editors and Affiliations

  1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, New South Wales, Australia

    Jeremy M. Crook

  2. WiCell Research Institute, Madison, Wisconsin, USA

    Tenneille E. Ludwig

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Chen, G., Rao, M. (2017). Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6921-0_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6919-7

  • Online ISBN: 978-1-4939-6921-0

  • eBook Packages: Springer Protocols

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