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Size Fractionation of Metal Species from Serum Samples for Studying Element Biodistribution in Alzheimer’s Disease

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Metals in the Brain

Part of the book series: Neuromethods ((NM,volume 124))

Abstract

This work considers the development of a novel analytical procedure for rapid and simple size fractionation of metal species from human serum samples, with a great potential for studying characteristic abnormalities of metal homeostasis associated with Alzheimer’s disease and other neurodegenerative disorders. For this purpose, serum samples are subjected to protein precipitation under non-denaturing conditions, taking special care for maintaining the integrity of metal–biomolecule bindings and for preventing species transformation, and then both the supernatant (low molecular mass fraction) and the precipitate (high molecular mass fraction) are analyzed by ICP-MS in order to determine the biodistribution of serum trace elements. This methodology is validated for 11 trace elements, including aluminum, cadmium, cobalt, chromium, copper, iron, manganese, molybdenum, selenium, vanadium, and zinc, in terms of sensitivity, selectivity, accuracy, and precision.

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Abbreviations

Aβ:

Amyloid β

AD:

Alzheimer’s disease

APP:

Amyloid precursor protein

CNS:

Central nervous system

CSF:

Cerebrospinal fluid

HMM:

High molecular mass

ICP-MS:

Inductively coupled plasma mass spectrometry

LMM:

Low molecular mass

LOD:

Limit of detection

MCI:

Mild cognitive impairment

PCA:

Principal component analysis

PLS-DA:

Partial least squares discriminant analysis

RSD:

Relative standard deviation

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  1. Faculty of Experimental Sciences, Department of Chemistry and Materials Science, University of Huelva, Campus de El Carmen, Avenue of the Armed Forces, 21007, Huelva, Spain

    Raúl González-Domínguez

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  1. Raúl González-Domínguez
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Correspondence to Raúl González-Domínguez .

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  1. Department of Pathology, University of Melbourne, Melbourne, Victoria, Australia

    Anthony R. White

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González-Domínguez, R. (2017). Size Fractionation of Metal Species from Serum Samples for Studying Element Biodistribution in Alzheimer’s Disease. In: White, A. (eds) Metals in the Brain. Neuromethods, vol 124. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6918-0_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6918-0_8

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