Abstract
Aptamers are single-stranded RNA or DNA oligonucleotides which have specific three-dimensional (3D) structures for high affinity and specific recognition to their target. In diagnostic and detection assays, aptamers represent an alternative to antibodies as recognition agents and offer advantages, such as reduced cost, rapid and reproducible synthesis, controllable modification, and improved stability. Aptamers are favorably used in biosensors as sensitive and selective bio-receptors coupled with a variety of transducers such as optical, mass-sensitive, and electrochemical sensors, the so-called aptasensors. We report the development of several types of aptasensors for rapid and specific detection of avian influenza virus (AIV) H5N1. DNA aptamers with high affinity and specificity against AIV H5N1 were immobilized on the electrode surface and then incorporated into different transducers such as surface plasmon resonance (SPR), quartz crystal microbalance (QCM) and electrochemical transducer. The target viruses were captured by the immobilized aptamers resulting in a detectable signal. The fabrication of aptasensors, detection principles, and their applications for AIV H5N1 detection are addressed.
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References
WHO, 2016. Cumulative number of confirmed human cases of avian influenza A(H5N1) reported to WHO, 2003–2015. January 20, 2016. http://www.who.int/influenza/human_animal_interface/2016_01_20_tableH5N1.pdf
Chen J, Jin M, Yu Z, Dan H, Zhang A, Song Y, Chen H (2007) A latex agglutination test for the rapid detection of avian influenza virus subtype H5N1 and its clinical application. J Vet Diagn Invest 19(2):155–160
Xie Z, Pang Ys, Liu J, Deng X, Tang X, Sun J, Khan MI (2006) A multiplex RT-PCR for detection of type A influenza virus and differentiation of avian H5, H7, and H9 hemagglutinin subtypes. Mol Cell Probes 20(3–4):245–249
Drexler JF, Helmer A, Kirberg H, Reber U, Panning M, Müller M, Höfling K, Matz B, Drosten C, Eis-Hübinger AM (2009) Poor clinical sensitivity of rapid antigen test for influenza A pandemic (H1N1) 2009 virus. Emerg Infect Dis 15(10):1662–1664
He Q, Velumani S, Du Q, Lim CW, Ng FK, Donis R, Kwang J (2007) Detection of H5 avian influenza viruses by antigen-capture enzyme-linked immunosorbent assay using H5-specific monoclonal antibody. Clin Vaccine Immunol 14(5):617–623
Jayasena SD (1999) Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin Chem 45(9):1628–1650
Bai H, Wang R, Hargis B, Lu H, Li Y (2012) A SPR aptasensor for detection of avian influenza virus H5N1. Sensors (Basel) 12(9):12506–12518
Wang R, Li Y (2013) Hydrogel based QCM aptasensor for detection of avian influenza virus. Biosens Bioelectron 42:148–155
Lum J, Wang R, Hargis B, Tung S, Bottje W, Lu H, Li Y (2015) An impedance aptasensor with microfluidic chips for specific detection of H5N1 avian influenza virus. Sensors (Basel) 15(8):18565–18578
Fu Y, Callaway Z, Lum J, Wang R, Lin J, Li Y (2014) Exploiting enzyme catalysis in ultra-low ion strength media for impedance biosensing of avian influenza virus using a bare interdigitated electrode. Anal Chem 86(4):1965–1971
Wang R, Xu L, Li Y (2015) Bio-nanogate controlled enzymatic reaction for virus sensing. Biosens Bioelectron 67:400–407
Wang R, Zhao J, Jiang T, Kwon YM, Lu H, Jiao P, Liao M, Li Y (2013) Selection and characterization of DNA aptamers for use in detection of avian influenza virus H5N1. J Virol Methods 189(2):362–369
Varshney M, Li Y, Srinivasan B, Tung S (2007) A label-free, microfluidics and interdigitated array microelectrode-based impedance biosensor in combination with nanoparticles immunoseparation for detection of Escherichia coli O157:H7 in food samples. Sens Actuators B 128:99–107
Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys Sci 1973(241):20–22
Zhou L, Jiang Y, Gao J, Zhao X, Ma L, Zhou Q (2012) Oriented immobilization of glucose oxidase on graphene oxide. Biochem Eng J 69:28–31
Wang R, Tombelli S, Minunni M, Spiriti MM, Mascini M (2004) Immobilisation of DNA probes for the development of SPR-based sensing. Biosens Bioelectron 20(5):967–974
Homola J (2003) Present and future of surface plasmon resonance biosensors. Anal Bioanal Chem 377:528–539
Acknowledgments
This research was supported in part by grants from USDA/NIFA (2009-35603-05063) and Arkansas Biosciences Institute (0383-43052-24-2099). The authors thank Dr. Billy Hargis, Dr. Steve Tung, Dr. Huaguang Lu, Dr. Young-Min Kwon, Dr. Walter Bottje, Dr. Ming Liao, Dr. Peirong Jiao, Dr. Yingchun Fu, Dr., Tieshan Jiang, Dr. Jacob Lum, Zach Callaway, Hua Bai, Jingjing Zhao, and Lizhou Xu for their contributions to this research and the related journal articles published.
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Li, Y., Wang, R. (2017). Aptasensors for Detection of Avian Influenza Virus H5N1. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_25
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DOI: https://doi.org/10.1007/978-1-4939-6911-1_25
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