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Dynamic Antibiotic Susceptibility Test via a 3D Microfluidic Culture Device

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Biosensors and Biodetection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1572))

Abstract

This study presents a novel microfluidic approach to the investigation of bacterial susceptibility against antibiotics gradient concentration in a quantitative way. A linear concentration gradient of antibiotics in a microfluidic chamber is used with time-lapse photography. This approach provides high-resolution information in real time. Traditional tests for this type of study involve Clinical and Laboratory Standard Institute (CLSI) recommended test and Etest. All of these tests need different trials to present different concentrations. They can only provide either static data or few sampling points in a time-consuming way. Our approach employes a gradient generated structure to perform thousands of trials in parallel, showing the approach can give results in rapidly, efficiently and reveal the dynamics.

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Author information

Authors and Affiliations

  1. Department of Engineering Sciences, Microsystem Technology, Uppsala University, The Angstrom Laboratory, Box 534, 751 21, Uppsala, Sweden

    Zining Hou, Yu An & Zhigang Wu

  2. School of Life Science, Fudan University, 200433, Shanghai, China

    Zining Hou & Yu An

  3. State Key Laboratory of Digital Manufacturing Equipment and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Zhigang Wu

Authors
  1. Zining Hou
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  2. Yu An
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  3. Zhigang Wu
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Corresponding author

Correspondence to Zhigang Wu .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

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Cite this protocol

Hou, Z., An, Y., Wu, Z. (2017). Dynamic Antibiotic Susceptibility Test via a 3D Microfluidic Culture Device. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_24

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  • DOI: https://doi.org/10.1007/978-1-4939-6911-1_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6910-4

  • Online ISBN: 978-1-4939-6911-1

  • eBook Packages: Springer Protocols

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