Abstract
MicroScale Thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here, we describe the application of MST in quantifying two CBM-carbohydrate interactions, a CBM3a toward cellulose nanocrystals and a CBM4 against xylohexaose.
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Acknowledgment
We acknowledge the Fédération de Recherche Agrobiosciences, Interactions et Biodiversité (FR 3450), CNRS, Université de Toulouse, UPS, Castanet-Tolosan, France, and the IDEX “UNITI” Université de Toulouse (GO-AHEAD project) for the Nanotemper Monolith NT.115 facilities.
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Wu, H., Montanier, C.Y., Dumon, C. (2017). Quantifying CBM Carbohydrate Interactions Using Microscale Thermophoresis. In: Abbott, D., Lammerts van Bueren, A. (eds) Protein-Carbohydrate Interactions. Methods in Molecular Biology, vol 1588. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6899-2_10
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DOI: https://doi.org/10.1007/978-1-4939-6899-2_10
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