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Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA

  • Hidetoshi Tahara
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1587)

Abstract

Both telomere length and telomere G-tail length are altered in human diseases such as cancer and age-related diseases. While most methods for measurement of G-tail and telomere length require electrophoresis, centrifugation, radioisotope labeling and autoradiography, G-tail telomere HPA provides a convenient and useful tool for the examination of G-tail length with a high-throughput platform using genomic DNA or cell lysate. G-tail telomere HPA may be applicable for clinical diagnostics as well as drug target screening.

Key words

G-Tail HPA; hybridization protection assay High-throughput Risk assessment Cancer Aging 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Cellular and Molecular Biology, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan

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