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High-Throughput Production of Proteins in E. coli for Structural Studies

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Heterologous Gene Expression in E.coli

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1586))

Abstract

We have developed a standardized and efficient workflow for high-throughput (HT) protein expression in E. coli and parallel purification which can be tailored to the downstream application of the target proteins. It includes a one-step purification for the purposes of functional assays and a two-step protocol for crystallographic studies, with the option of on-column tag removal.

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Author information

Authors and Affiliations

  1. Evotec Ltd, 114 Innovation Drive, Milton Park, Abingdon, Oxfordshire, OX14 4RZ, UK

    Charikleia Black, John J. Barker, Richard B. Hitchman, Hok Sau Kwong & Sam Festenstein

  2. Evotec (U.S.) Inc., Building 303B, College Road East, Princeton, NJ, 08540, USA

    Thomas B. Acton

Authors
  1. Charikleia Black
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  2. John J. Barker
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  3. Richard B. Hitchman
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  4. Hok Sau Kwong
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  5. Sam Festenstein
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  6. Thomas B. Acton
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Corresponding author

Correspondence to John J. Barker .

Editor information

Editors and Affiliations

  1. SGC, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom

    Nicola A. Burgess-Brown

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Black, C., Barker, J.J., Hitchman, R.B., Kwong, H.S., Festenstein, S., Acton, T.B. (2017). High-Throughput Production of Proteins in E. coli for Structural Studies. In: Burgess-Brown, N. (eds) Heterologous Gene Expression in E.coli. Methods in Molecular Biology, vol 1586. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6887-9_24

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  • DOI: https://doi.org/10.1007/978-1-4939-6887-9_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6885-5

  • Online ISBN: 978-1-4939-6887-9

  • eBook Packages: Springer Protocols

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