Abstract
T cells are the main cellular targets of the human immunodeficiency virus 1 (HIV-1). HIV-1 infection induces pleiotropic effects on the infected T cell that modify the T cell capacity to respond to antigen and facilitates virus replication. HIV-1 infection subverts the formation and function of the immunological synapse altering both actin cytoskeleton remodeling and intracellular vesicle traffic. We describe here our methods to unveil how HIV-1 and in particular its protein Nef modify vesicle traffic to the immunological synapse, perturbing the synapse activation capacity.
*These authors contributed equally to this work.
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Acknowledgments
This work was supported by grants from Agence Nationale de la Recherche (ANR, No. 11 BSV3 025 01), Agence National de Recherche sur le SIDA et les Hepatitis Virales (ANRS), Institut Pasteur, CNRS, and INSERM. IdRI is a scholar in the Pasteur-Paris University (PPU) International PhD program and is funded by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme FP7/2007–2013/under the REA grant agreement n° 317057 HOMIN. J.B. was supported by ANRS and Roux-Institut Pasteur postdoctoral fellowships. The following reagents were obtained through the NIH AIDS Reagent Program, AIDS Program, NIAID, and NIH: Anti-HIV-1 JR-CSF Nef Monoclonal (6.2) from Dr. K. Krohn and Dr. V. Ovod [14] and anti-HIV-1 SF2 p24 polyclonal antibody and human rIL-2 from Dr. M. Gately, Hoffmann-La Roche Inc.
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del Río-Iñiguez, I., Bouchet, J., Alcover, A. (2017). Studying the Immune Synapse in HIV-1 Infection. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_34
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DOI: https://doi.org/10.1007/978-1-4939-6881-7_34
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