Abstract
In this chapter, we describe the technical details to visualize and analyze effector immunological synapses between T cells and astrocytes in the brain with high-resolution confocal imaging. This procedure is critical for the optimal and even penetration of labeling antibodies within the nerve tissue to obtain accurate staining and allow a uniform three-dimensional analysis of the T cell-astrocyte interactions. We emphasize here the comprehensive exploration of the tissue and analysis with confocal microscope as well as the display of microanatomical details of the three-dimensional reconstruction for interface visualization (including peripheral and central supramolecular activation clusters, effector molecules, and other organelles such as microtubule organizing centers (MTOCs) and Golgi apparatus).
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Cribaro, G.P., Saavedra-López, E., Casanova, P.V., Rodríguez, L., Barcia, C. (2017). Studying the T Cell-Astrocyte Immune Synapse. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_32
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DOI: https://doi.org/10.1007/978-1-4939-6881-7_32
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6879-4
Online ISBN: 978-1-4939-6881-7
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