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The Immune Synapse pp 291–306Cite as

Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1584))

Abstract

The immune synapse has emerged as a compelling example of structural complexity within cell-cell interfaces. This chapter focuses on the use of microcontact printing to isolate and investigate how spatial organization of signaling molecules drives the function of immune cells. In the process detailed here, multiple rounds of microcontact printing are combined to create patterned surfaces that control the relative spatial localization of CD3 and CD28 signaling in T cells, effectively replacing an antigen presenting cell with an engineered surface. A set of approaches used to address key issues of T cell activation are described and discussed.

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Acknowledgments

This work was supported by the National Institutes of Health, R01AI088377 and U24AI118669.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Columbia University, 120 Amsterdam Ave Mail Code 8904, New York, NY, 10027, USA

    Joung-Hyun Lee & Lance C. Kam

Authors
  1. Joung-Hyun Lee
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  2. Lance C. Kam
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Corresponding author

Correspondence to Lance C. Kam .

Editor information

Editors and Affiliations

  1. Department of Life sciences, University of Siena, Siena, Siena, Italy

    Cosima T. Baldari

  2. University of Oxford, Kennedy Institute of Rheumatology, Headington, Oxford, United Kingdom

    Michael L. Dustin

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Lee, JH., Kam, L.C. (2017). Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6881-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6879-4

  • Online ISBN: 978-1-4939-6881-7

  • eBook Packages: Springer Protocols

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