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Characterization of Th9 Cells in the Development of EAE and IBD

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Th9 Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1585))

Abstract

Encephalitogenic and colitogenic effector T cells have been implicated in the induction of experimental autoimmune encephalomyelitis (EAE) and inflammatory bowel disease (IBD), respectively. Effector functions of Th1 and Th17 cells have been well characterized and described for the induction and development of EAE and IBD; however, the recently identified Th9 cells have also been suggested to play an important role in these autoimmune pathologies. Th9 cells, primarily characterized by their high level of production of IL-9, are not only essential in controlling extracellular pathogens but also contribute to the development of autoimmunity and allergic inflammation. Furthermore, it was also demonstrated that IL-9 promotes Th17 cell-mediated tissue pathology in EAE and it compromises the barrier functions of the gut in IBD. In vivo adoptive transfer of in vitro differentiated Th9 cells induces the development of autoimmune tissue inflammation in EAE and IBD. Here we describe methods for in vitro differentiation of naïve murine CD4+ T cells to generate IL-9-producing Th9 cells and follow their effector functions in EAE and IBD murine models.

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Acknowledgment

This work was supported by grant from Department of Biotechnology (DBT), Govt. of India and DBT-Wellcome Trust intermediate fellowship (IA/I/12/1/500524). S.M. is supported by a senior research fellowship from Council of Scientific and Industrial Research (CSIR), Govt of India. V.D. is supported by CNRS.

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Authors and Affiliations

  1. Center for Human Microbial Ecology (CHME), Translational Health Science & Technology Institute (THTI), 3rd Milestone, Gurgaon-Faridabad Expressway, Faridabad, 121 001, Haryana, India

    Sakshi Malik & Amit Awasthi Ph.D.

  2. Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique UMR5535, Université de Montpellier, 34293, Montpellier, France

    Valerie Dardalhon

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  1. Sakshi Malik
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  2. Valerie Dardalhon
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  3. Amit Awasthi Ph.D.
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Correspondence to Amit Awasthi Ph.D. .

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Editors and Affiliations

  1. School of Bio Science, Sir JC Bose Laboratory Complex, Indian Institute of Technology, Kharagpur, West Bengal, India

    Ritobrata Goswami

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Malik, S., Dardalhon, V., Awasthi, A. (2017). Characterization of Th9 Cells in the Development of EAE and IBD. In: Goswami, R. (eds) Th9 Cells. Methods in Molecular Biology, vol 1585. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6877-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6877-0_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6876-3

  • Online ISBN: 978-1-4939-6877-0

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