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Th9 Cells pp 127–140Cite as

Flow Cytometric Assessment of STAT Molecules in Th9 Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1585))

Abstract

IL-9-producing Th9 cells are a novel subset of T helper cells that develop independently of other T helper subsets. Th9 cells have been implicated in the pathogenesis of allergic asthma and autoimmunity, while also serving as critical effector T cells in mediating antitumor immune responses. Concomitant presence of TGF-β and IL-4 lead to the differentiation of naïve CD4+ T cells towards the Th9 phenotype. In addition, several cytokines, including IL-1β, IL-2, IL-25, and IL-33, further amplify Th9 responses. Negative regulators of Th9 cells include other cytokines such as IFN-γ, IL-23, and IL-27. Here, we describe a detailed protocol for the analysis of STAT molecules involved in the differentiation of Th9 cells and Th9 inhibition by IL-27.

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Acknowledgments

This work was supported by grants from the National Multiple Sclerosis Society (RG 4904A2/1 and RG 1507-05164).

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Authors and Affiliations

  1. Ann Romney Center for Neurologic Diseases, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA

    Lucien P. Garo, Vanessa Beynon & Gopal Murugaiyan

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  1. Lucien P. Garo
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  2. Vanessa Beynon
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  3. Gopal Murugaiyan
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Corresponding author

Correspondence to Gopal Murugaiyan .

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Editors and Affiliations

  1. School of Bio Science, Sir JC Bose Laboratory Complex, Indian Institute of Technology, Kharagpur, West Bengal, India

    Ritobrata Goswami

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Garo, L.P., Beynon, V., Murugaiyan, G. (2017). Flow Cytometric Assessment of STAT Molecules in Th9 Cells. In: Goswami, R. (eds) Th9 Cells. Methods in Molecular Biology, vol 1585. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6877-0_10

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  • DOI: https://doi.org/10.1007/978-1-4939-6877-0_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6876-3

  • Online ISBN: 978-1-4939-6877-0

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