Abstract
Cholesterol levels are under tight control within cells. This involves a complex interplay of balancing synthesis, uptake, and export. A major player in the transcriptional regulation of cholesterol levels is sterol regulatory element binding protein (SREBP). SREBP is upregulated in conditions of low cholesterol, and then binds to sterol regulatory elements (SREs) that exist within the promoters of genes involved in cholesterol synthesis and uptake.
Here, we describe a method to identify sterol response elements (SREs) using in silico and experimental approaches.
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References
Horton JD, Shah NA, Warrington JA, Anderson NN, Park SW, Brown MS, Goldstein JL (2003) Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes. Proc Natl Acad Sci 100:12027–12032
Sharpe LJ, Brown AJ (2013) Controlling cholesterol synthesis beyond 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). J Biol Chem 288:18707–18715
Zerenturk EJ, Sharpe LJ, Brown AJ (2012) Sterols regulate 3beta-hydroxysterol Delta24-reductase (DHCR24) via dual sterol regulatory elements: Cooperative induction of key enzymes in lipid synthesis by Sterol Regulatory Element Binding Proteins. Biochim Biophys Acta 1821:1350–1360
Prabhu AV, Sharpe LJ, Brown AJ (2014) The sterol-based transcriptional control of human 7-dehydrocholesterol reductase (DHCR7): Evidence of a cooperative regulatory program in cholesterol synthesis. Biochim Biophys Acta 1842:1431–1439
Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, Zahler AM, Haussler D (2002) The human genome browser at UCSC. Genome Res 12:996–1006
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Sharpe, L.J., Brown, A.J. (2017). Identifying Sterol Response Elements Within Promoters of Genes. In: Gelissen, I., Brown, A. (eds) Cholesterol Homeostasis. Methods in Molecular Biology, vol 1583. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6875-6_13
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DOI: https://doi.org/10.1007/978-1-4939-6875-6_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6873-2
Online ISBN: 978-1-4939-6875-6
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