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Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1582))

Abstract

Cytofluorimetric analysis is a typical method in immunology to evaluate phenotype and function of Natural Killer (NK) cells derived from HTLV-1/2 infected patients and healthy donors. Here, we described protocols to NK cells phenotypical and cytotoxicity assay, performed by flow cytometry on fresh and immune-magnetically or flow cytometry sorted NK cells. A new developed protocol able to evaluate IFNγ production has been included.

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Correspondence to Andrea De Maria .

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Bozzano, F., Marras, F., De Maria, A. (2017). Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection. In: Casoli, C. (eds) Human T-Lymphotropic Viruses. Methods in Molecular Biology, vol 1582. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6872-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6872-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6870-1

  • Online ISBN: 978-1-4939-6872-5

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