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Recombinant Virus Vaccines pp 133–149Cite as

Development of Recombinant Arenavirus-Based Vaccines

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1581))

Abstract

The development of arenavirus reverse genetics has provided investigators with a novel and powerful approach for the investigation of the arenavirus molecular and cell biology. The use of cell-based minigenome systems has allowed examining the cis- and trans-acting factors involved in arenavirus replication and transcription, and the identification of novel anti-arenaviral drug targets without requiring the use of live forms of arenaviruses. Likewise, it is now feasible to rescue infectious arenaviruses entirely from cloned cDNAs containing predetermined mutations in their genomes to investigate virus-host interactions and mechanisms of pathogenesis. These advances in arenavirus genetics have also facilitated screens to identify anti-arenaviral drugs and the pursuit of novel strategies to generate live-attenuated arenavirus vaccine candidates. Moreover, the generation of tri-segmented (r3) arenaviruses expressing foreign genes of interest (GOI) has opened the possibility of implementing live-attenuated arenaviruses-based vaccine vector approaches. In this chapter, we will summarize the implementation of plasmid-based reverse genetics techniques for the development of r3 arenaviruses expressing foreign GOI for their implementation as vaccine vectors.

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Acknowledgments

Current arenavirus research in LM-S laboratory is funded by the NIH grants R21 AI119775-01, R43 AI119775-01, and R21AI121550-01A1. Research in J.C.T. laboratory is supported by grants RO1 AI047140, RO1 AI077719, and RO1 AI079665.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA

    Luis Martínez-Sobrido

  2. Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, 92037, USA

    Juan Carlos de la Torre

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  1. Luis Martínez-Sobrido
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  2. Juan Carlos de la Torre
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Correspondence to Luis Martínez-Sobrido or Juan Carlos de la Torre .

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  1. Rochester Institute of Technology, Thomas H. Gosnell School of Life Sciences, Rochester, New York, USA

    Maureen C. Ferran

  2. Rochester Institute of Technology, Thomas H. Gosnell School of Life Sciences, Rochester, New York, USA

    Gary R. Skuse

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Martínez-Sobrido, L., de la Torre, J.C. (2017). Development of Recombinant Arenavirus-Based Vaccines. In: Ferran, M., Skuse, G. (eds) Recombinant Virus Vaccines. Methods in Molecular Biology, vol 1581. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6869-5_8

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