Abstract
Pseudorabies virus (PRV) is a promising vaccine vector due to its distinctive features including many nonessential replication regions and a broad host range. Foreign genes of other viruses have been successfully inserted into and expressed in PRV and these recombinant viruses are very likely to induce humoral and/or cellular responses in immunized animals. This chapter offers an overview of methods for generating recombinant pseudorabies virus for use as a vaccine vector.
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Acknowledgments
This work was supported by grant from Innovation Scientists and Technicians Troop Construction Projects of Henan Province (Grant No.142101510001), Talents Plan for Scientific and Technological Innovation in Henan Province (Grant No.144200510002), Science and Technology Innovation team in Henan Province (Team No. C20130005), and Luoyang Heluo Talent Plan (Kegong Tian).
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Tan, F., Li, X., Tian, K. (2017). Generating Recombinant Pseudorabies Virus for Use as a Vaccine Platform. In: Ferran, M., Skuse, G. (eds) Recombinant Virus Vaccines. Methods in Molecular Biology, vol 1581. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6869-5_5
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DOI: https://doi.org/10.1007/978-1-4939-6869-5_5
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