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Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum

  • Thorarinn Blondal
  • Maurizia Rossana Brunetto
  • Daniela Cavallone
  • Martin Mikkelsen
  • Michael Thorsen
  • Yuan Mang
  • Hazel Pinheiro
  • Ferruccio Bonino
  • Peter MouritzenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1580)

Abstract

This study compares next-generation sequencing (NGS) technologies that have been optimized specifically for biofluid samples, with more established qPCR-based methods for profiling microRNAs in biofluids. The same patient serum samples were analyzed by NGS and qPCR, and differences in the serum microRNA profile between HBV and HCV infected patients were investigated. While there was overall good agreement between NGS and qPCR, there were some differences between the platforms, highlighting the importance of validation.

Key words

microRNA miRNA Next-generation sequencing NGS qPCR Real-time PCR Biofluids Serum Plasma Platform comparison HBV HCV Liquid biopsy Profiling Validation 

Notes

Acknowledgment

We thank Jannie Rosendahl Christensen, Tine Charlotte Sandberg, and Efat Sadat for excellent technical assistance.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Thorarinn Blondal
    • 1
  • Maurizia Rossana Brunetto
    • 2
  • Daniela Cavallone
    • 2
  • Martin Mikkelsen
    • 1
  • Michael Thorsen
    • 1
  • Yuan Mang
    • 1
  • Hazel Pinheiro
    • 1
  • Ferruccio Bonino
    • 2
  • Peter Mouritzen
    • 1
    Email author
  1. 1.Exiqon A/SVedbaekDenmark
  2. 2.Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and CancerUniversity Hospital of PisaPisaItaly

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