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MicroRNA Detection and Target Identification pp 21–44Cite as

Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1580))

Abstract

This study compares next-generation sequencing (NGS) technologies that have been optimized specifically for biofluid samples, with more established qPCR-based methods for profiling microRNAs in biofluids. The same patient serum samples were analyzed by NGS and qPCR, and differences in the serum microRNA profile between HBV and HCV infected patients were investigated. While there was overall good agreement between NGS and qPCR, there were some differences between the platforms, highlighting the importance of validation.

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Acknowledgment

We thank Jannie Rosendahl Christensen, Tine Charlotte Sandberg, and Efat Sadat for excellent technical assistance.

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Authors and Affiliations

  1. Exiqon A/S, Vedbaek, Denmark

    Thorarinn Blondal, Martin Mikkelsen, Michael Thorsen, Yuan Mang, Hazel Pinheiro & Peter Mouritzen

  2. Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy

    Maurizia Rossana Brunetto, Daniela Cavallone & Ferruccio Bonino

Authors
  1. Thorarinn Blondal
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  2. Maurizia Rossana Brunetto
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  3. Daniela Cavallone
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  4. Martin Mikkelsen
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  5. Michael Thorsen
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  6. Yuan Mang
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  7. Hazel Pinheiro
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  8. Ferruccio Bonino
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  9. Peter Mouritzen
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Corresponding author

Correspondence to Peter Mouritzen .

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Editors and Affiliations

  1. School of Biological Sciences, University of East Anglia, Norwich, United Kingdom

    Tamas Dalmay

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Blondal, T. et al. (2017). Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum. In: Dalmay, T. (eds) MicroRNA Detection and Target Identification. Methods in Molecular Biology, vol 1580. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6866-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6866-4_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6864-0

  • Online ISBN: 978-1-4939-6866-4

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