Abstract
The use of fluorescent probes in conjunction with white-light colonoscopy is a promising strategy for improving the detection of precancerous colorectal lesions, in particular flat (sessile) lesions that do not protrude into the lumen of the colon. We describe a method for determining the sensitivity and specificity of an enzymatically activated near-infrared probe (MMPSense680) for the detection of colon lesions in a mouse model (APC+/Min-FCCC) of spontaneous colorectal cancer. Fluorescence intensity correlates directly with the activity of matrix metalloproteinases (MMPs). Overexpression of MMPs is an early event in the development of colorectal lesions. Although the probe employed serves as a reporter of the activity of MMPs, our method can be applied to any fluorescent probe that targets an early molecular event in the development of colorectal tumors.
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References
Cornett D, Barancin C, Roeder B et al (2008) Findings on optical colonoscopy after positive CT colonography exam. Am J Gastroenterol 103:2068–2074
Heresbach D, Barrioz T, Lapalus MG et al (2008) Miss rate for colorectal neoplastic polyps: a prospective multicenter study of back-to-back video colonoscopies. Endoscopy 40:284–290
Burggraaf J, Kamerling IM, Gordon PB et al (2015) Detection of colorectal polyps in humans using an intravenously administered fluorescent peptide targeted against c-Met. Nat Med 21:955–961
Clapper ML, Hensley HH, Chang WC et al (2011) Detection of colorectal adenomas using a bioactivatable probe specific for matrix metalloproteinase activity. Neoplasia 13:685–691
Cooper HS, Chang WC, Coudry R et al (2005) Generation of a unique strain of multiple intestinal neoplasia (Apc(+/Min-FCCC)) mice with significantly increased numbers of colorectal adenomas. Mol Carcinog 44:31–41
Weissleder R, Ntziachristos V (2003) Shedding light onto live molecular targets. Nat Med 9:123–128
Wagenaar-Miller RA, Gorden L, Matrisian LM (2004) Matrix metalloproteinases in colorectal cancer: is it worth talking about? Cancer Metastasis Rev 23:119–135
Zucker S, Vacirca J (2004) Role of matrix metalloproteinases (MMPs) in colorectal cancer. Cancer Metastasis Rev 23:101–117
Newell KJ, Witty JP, Rodgers WH et al (1994) Expression and localization of matrix-degrading metalloproteinases during colorectal tumorigenesis. Mol Carcinog 10:199–206
Kinzler KW, Vogelstein B (1996) Lessons from hereditary colorectal cancer. Cell 87:159–170
Acknowledgments
This work was supported by grants CA-006927 and CA-124693 from the National Cancer Institute, by Fox Chase Cancer Center Keystone Initiative in Personalized Risk and Prevention, and by an appropriation from the Commonwealth of Pennsylvania. The authors have no conflicts of interest or financial disclosures to report.
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Hensley, H., Cooper, H.S., Chang, WC.L., Clapper, M.L. (2017). Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology. In: Galea, C. (eds) Matrix Metalloproteases. Methods in Molecular Biology, vol 1579. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6863-3_13
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DOI: https://doi.org/10.1007/978-1-4939-6863-3_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6861-9
Online ISBN: 978-1-4939-6863-3
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