Abstract
The use of fluorescent probes in conjunction with white-light colonoscopy is a promising strategy for improving the detection of precancerous colorectal lesions, in particular flat (sessile) lesions that do not protrude into the lumen of the colon. We describe a method for determining the sensitivity and specificity of an enzymatically activated near-infrared probe (MMPSense680) for the detection of colon lesions in a mouse model (APC+/Min-FCCC) of spontaneous colorectal cancer. Fluorescence intensity correlates directly with the activity of matrix metalloproteinases (MMPs). Overexpression of MMPs is an early event in the development of colorectal lesions. Although the probe employed serves as a reporter of the activity of MMPs, our method can be applied to any fluorescent probe that targets an early molecular event in the development of colorectal tumors.
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Acknowledgments
This work was supported by grants CA-006927 and CA-124693 from the National Cancer Institute, by Fox Chase Cancer Center Keystone Initiative in Personalized Risk and Prevention, and by an appropriation from the Commonwealth of Pennsylvania. The authors have no conflicts of interest or financial disclosures to report.
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Hensley, H., Cooper, H.S., Chang, WC.L., Clapper, M.L. (2017). Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology. In: Galea, C. (eds) Matrix Metalloproteases. Methods in Molecular Biology, vol 1579. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6863-3_13
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DOI: https://doi.org/10.1007/978-1-4939-6863-3_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6863-3
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