Abstract
Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis. They are also involved in the pathogenesis of a vast array of diseases, including but not limited to systemic inflammation, various cancers, and cardiovascular, neurological, and autoimmune diseases. Here, we describe gel zymography to detect MMPs in cell and tissue samples and in cell culture supernatants.
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Acknowledgment
This work was supported in part by funding from Baylor College of Medicine and National Institutes of Health grants AR059838 and NS073712 to C.B.
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Tajhya, R.B., Patel, R.S., Beeton, C. (2017). Detection of Matrix Metalloproteinases by Zymography. In: Galea, C. (eds) Matrix Metalloproteases. Methods in Molecular Biology, vol 1579. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6863-3_12
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DOI: https://doi.org/10.1007/978-1-4939-6863-3_12
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