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Assays to Investigate the N-Glycosylation State and Function of Plant Pattern Recognition Receptors

  • Stacey A. Lawrence
  • Teresa Ceserani
  • Nicole K. ClayEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

The biogenesis and functionality of pattern recognition receptors (PRRs) are critical for robust plant immune responses. Here, we present methods to determine the N-glycosylation state and ligand-induced activity of these receptors for comparative quantitative analysis. These techniques can be used to identify mutants and chemical inhibitors affecting PRR biogenesis and functionality. When combined, these techniques can provide useful insights on biological processes necessary to synthesize a properly membrane-localized and ligand-responsive PRR.

Key words

Asparagine-linked glycosylation Protein quality control Endoglycosidase H Tunicamycin MAPK activation Seedling growth arrest Anthocyanin inhibition 

Notes

Acknowledgments

This work was supported by T32 GM007499 (to S.A.L.) and Yale University Elizabeth Brown Postdoctoral Fellowship (to T.C.).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Stacey A. Lawrence
    • 1
  • Teresa Ceserani
    • 1
  • Nicole K. Clay
    • 1
    Email author
  1. 1.Department of Molecular, Cellular & Developmental BiologyYale UniversityNew HavenUSA

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