Methods to Quantify PAMP-Triggered Oxidative Burst, MAP Kinase Phosphorylation, Gene Expression, and Lignification in Brassicas
Quantitative disease resistance (QDR) based on PAMP-triggered immunity (PTI) could be durable and effective against many pathogens (broad spectrum). Development of methods to evaluate PTI responses in crops could therefore accelerate breeding for durable QDR. Most PTI-studies involved model plants such as Arabidopsis and Nicotiana benthamiana or cell cultures, and cannot be directly applied to diverse germplasm of crop plants.
We developed methods to measure PTI in Brassica crop species (Lloyd et al., Mol Plant Microbe Interact 27:286–295, 2014) which we have elaborated and expanded here to enable their use for screening and evaluating germplasm for potential QDR in breeding programs.
Key wordsPAMP-triggered immunity Crop plants Brassica napus Brassica oleracea Quantitative disease resistance Reactive oxygen species Lignin
We like to thank Sam Holt and Francesca Stefanato for critical reading of the manuscript, Cecile Ségonzac and Freddy Boutrot in the Cyril Zipfel group (The Sainsbury Lab, Norwich, UK) for sharing their PTI protocols and thinking about adaptations for use on Brassica plants, and Yaizu Suisankagaku Industry CO (Yaizu, Japan) for providing CSC. This work was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/G042960/1 (as part of the consortium ERA-PG “PRR-CROP”), BB/N005007/1 (as part of the ERA-CAPS consortium “MAQBAT”), the John Innes Institute BIO strategic grant BB/J004553/1 and by a BBSRC doctoral training grant to S.R.L.
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