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Chitin-Induced Responses in the Moss Physcomitrella patens

  • Simon Bressendorff
  • Magnus Wohlfahrt Rasmussen
  • Morten Petersen
  • John MundyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

A MAP kinase pathway below a chitin receptor in the moss Physcomitrella patens induces immune responses including rapid growth inhibition, a novel fluorescence burst, and cell wall depositions. The molecular mechanisms producing these three responses are currently unknown but warrant further investigation in this simple model system. Here we describe qualitative, time-lapse, and quantitative assays to monitor and measure these responses.

Key words

Bryophyte Plant innate immunity 

Notes

Acknowledgments

This work was supported by grants from the Danish Basic Research Foundation (Center for Comparative Genomics) and the Danish Research Council for Nature and the Universe (Comparative and functional genomics of plant innate immunity, 1323-00267A) to J.M.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Simon Bressendorff
    • 1
  • Magnus Wohlfahrt Rasmussen
    • 1
  • Morten Petersen
    • 1
  • John Mundy
    • 1
    Email author
  1. 1.Department of BiologyUniversity of CopenhagenCopenhagenDenmark

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