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Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells

  • Koji Yamaguchi
  • Tsutomu KawasakiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-cultured cells.

Key words

Immunity Chitin Rice MAPK ROS 

Notes

Acknowledgments

This research was supported by Grants-in-Aid for Scientific Research (A)(15H02489), for Scientific Research on Innovative Areas (25114517 and 15H01242), and by the Strategic Project to Support the Formation of Research Bases at Private Universities: Matching Fund Subsidy from the Ministry of Education, Culture, Sports, Science and Technology, 2011-2015 (S1101035) to T.K., and Grants-in-Aid for Scientific Research (15K18649) and Basic Science Research Projects from Sumitomo Foundation to K.Y.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Graduate School of AgricultureKindai UniversityNaraJapan

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