Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells
Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-cultured cells.
Key wordsImmunity Chitin Rice MAPK ROS
This research was supported by Grants-in-Aid for Scientific Research (A)(15H02489), for Scientific Research on Innovative Areas (25114517 and 15H01242), and by the Strategic Project to Support the Formation of Research Bases at Private Universities: Matching Fund Subsidy from the Ministry of Education, Culture, Sports, Science and Technology, 2011-2015 (S1101035) to T.K., and Grants-in-Aid for Scientific Research (15K18649) and Basic Science Research Projects from Sumitomo Foundation to K.Y.
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