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Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants

  • Jian HuaEmail author
  • Shuai Wang
  • Qi Sun
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

Here we describe a method of gene mapping and cloning of chemical induced mutations using next-generation sequencing on their backcrossed segregants. This method utilizes polymorphisms induced by chemical mutagens for mapping and therefore does not require outcrossing to a different background or a large segregating population. It can be used to clone causal mutations generated in various accessions or mutant backgrounds for dissecting complex processes such as pattern recognition receptor pathways in plant immunity.

Key words

Mapping Cloning Next-generation sequencing Mutagenesis 

Notes

Acknowledgment

This work was supported by National Science Foundation IOS–1353738 to J.H.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Plant Biology Section, School of Integrative Plant ScienceCornell UniversityIthacaUSA
  2. 2.Biocomputing Service Unit, Institute of BiotechnologyCornell UniversityIthacaUSA

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