Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants
Here we describe a method of gene mapping and cloning of chemical induced mutations using next-generation sequencing on their backcrossed segregants. This method utilizes polymorphisms induced by chemical mutagens for mapping and therefore does not require outcrossing to a different background or a large segregating population. It can be used to clone causal mutations generated in various accessions or mutant backgrounds for dissecting complex processes such as pattern recognition receptor pathways in plant immunity.
Key wordsMapping Cloning Next-generation sequencing Mutagenesis
This work was supported by National Science Foundation IOS–1353738 to J.H.