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Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment

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Plant Pattern Recognition Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1578))

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Abstract

Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.

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Acknowledgment

This research is supported by grants from USDA-NIFA (2014-67013-21554) and NSF-PGRP (IOS-1340001) for W. Ma.

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Authors and Affiliations

  1. Department of Plant Pathology and Microbiology, University of California, Riverside, CA, 92521, USA

    Yingnan Hou & Wenbo Ma

  2. Center for Plant Cell Biology, University of California, Riverside, CA, 92521, USA

    Yingnan Hou & Wenbo Ma

Authors
  1. Yingnan Hou
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  2. Wenbo Ma
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Corresponding author

Correspondence to Wenbo Ma .

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Editors and Affiliations

  1. Department of Plant Pathology and Microbiology Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas, USA

    Libo Shan

  2. Department of Biochemistry and Biophysics Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas, USA

    Ping He

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Hou, Y., Ma, W. (2017). Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment. In: Shan, L., He, P. (eds) Plant Pattern Recognition Receptors. Methods in Molecular Biology, vol 1578. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6859-6_23

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  • DOI: https://doi.org/10.1007/978-1-4939-6859-6_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6858-9

  • Online ISBN: 978-1-4939-6859-6

  • eBook Packages: Springer Protocols

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