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Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses

  • Laura Bacete
  • Hugo Mélida
  • Sivakumar Pattathil
  • Michael G. Hahn
  • Antonio Molina
  • Eva MiedesEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

The plant cell wall is one of the first defensive barriers that pathogens need to overcome to successfully colonize plant tissues. Plant cell wall is considered a dynamic structure that regulates both constitutive and inducible defense mechanisms. The wall is a potential source of a diverse set of Damage-Associated Molecular Patterns (DAMPs), which are signalling molecules that trigger immune responses. However, just a few active wall ligands, such as oligogalacturonic acids (OGs), have been characterized so far. To identify additional wall-derived DAMPs, we obtained different plant wall fractions and tested their capacity to trigger immune responses using a calcium read-out system. To characterize the active DAMPs structures present in these fractions, we applied Glycome Profiling, a technology that uses a large and diverse set of specific monoclonal antibodies against wall carbohydrate ligands. The methods describe here can be used in combination with other biochemical approaches to identify and purify new plant cell wall DAMPs.

Key words

Pectin Hemicellulose Cell wall Arabidopsis Immunity 

Notes

Acknowledgment

Research in the A. M. lab was financially supported by grants BIO2012-32910 and BIO2015-64077-R from Spanish Ministry of Economy and Competitiveness (MINECO), and in the M. G. H. lab (CCRC) by the United States NSF Plant Genome Program (DBI-0421683 and IOS-0923992). L. B. was a PhD fellow (BES-2013-065010) from MINECO and H. M. was supported by an IEF-SignWALLINg-624721 grant from the European Union.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Laura Bacete
    • 1
  • Hugo Mélida
    • 1
  • Sivakumar Pattathil
    • 2
  • Michael G. Hahn
    • 2
  • Antonio Molina
    • 1
  • Eva Miedes
    • 1
    Email author
  1. 1.Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)—Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Pozuelo de Alarcón (Madrid)Spain
  2. 2.Complex Carbohydrate Research Center (CCRC)University of GeorgiaAthensUSA

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