Network Reconstitution for Quantitative Subnetwork Interaction Analysis
A fundamental task in systems biology is to quantify the contributions of the systems’ parts and their interactions. Here I describe a powerful concept and tool for this purpose: network reconstitution. Genotypes of an organism that represent all possible combinations of the subnetworks in question will be quantitatively phenotyped. The quantitative phenotype data is analyzed using an R script to obtain estimates for single subnetwork contributions and their interactions.
Key wordsSubnetwork interactions Network reconstitution Signaling allocation Combinatorial genotypes Quantitative phenotype
I thank Rachel Hillmer for critical reading of the manuscript. This work was supported by grants from National Science Foundation, MCB-0918908, IOS-1121425, and MCB-1518058.