Chitin and Stress Induced Protein Kinase Activation
The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.
Key wordsAcryloylated peptide substrates In-gel assays Myelin basic protein
This work was supported by grants from the Danish Basic Research Foundation (Center for Comparative Genomics) and the Danish Research Council for Nature and the Universe (Comparative & functional genomics of plant innate immunity, 1323-00267A) to JM.
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