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Chitin and Stress Induced Protein Kinase Activation

  • Chandra Kenchappa
  • Raquel Azevedo da Silva
  • Simon Bressendorff
  • Sabrina Stanimirovic
  • Jakob Olsen
  • Morten Petersen
  • John MundyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

Key words

Acryloylated peptide substrates In-gel assays Myelin basic protein 

Notes

Acknowledgments

This work was supported by grants from the Danish Basic Research Foundation (Center for Comparative Genomics) and the Danish Research Council for Nature and the Universe (Comparative & functional genomics of plant innate immunity, 1323-00267A) to JM.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Chandra Kenchappa
    • 1
  • Raquel Azevedo da Silva
    • 1
  • Simon Bressendorff
    • 1
  • Sabrina Stanimirovic
    • 1
  • Jakob Olsen
    • 1
  • Morten Petersen
    • 1
  • John Mundy
    • 1
    Email author
  1. 1.Department of BiologyUniversity of CopenhagenCopenhagenDenmark

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