LeEIX2 Interactors’ Analysis and EIX-Mediated Responses Measurement
Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception. Here, we describe the in vivo co-IP methodology used for protein interaction verification and ethylene and ROS (reactive oxygen species) measurements used for physiological effects assessment.
Key wordsLRR-RLP Ethylene-inducing xylanase MAMP Defense responses Co-IP ROS
This work was partly supported by the Israel Science Foundation administered by the Israel Academy of Science and Humanities no. 388/12 and Research Grant Award no. IS-4842-15R from BARD, the United States–Israel Binational Agriculture Research and Development Fund and by the United States-Israel Binational Science Foundation no. 2013227.