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LeEIX2 Interactors’ Analysis and EIX-Mediated Responses Measurement

  • Meirav Leibman-Markus
  • Silvia Schuster
  • Adi AvniEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

Plant-pathogen interactions involve a large number of wide regulatory systems, necessary for plant defense responses against pathogen attack. The fungal protein ethylene-inducing xylanase (EIX) elicits defense responses in specific cultivars of tobacco and tomato. The response to EIX is controlled by a single locus encoding for LeEIX2, a leucine-rich-repeat receptor-like-protein (LRR-RLP). As an RLP, LeEIX2 does not possess an obvious cytoplasmic signaling moiety such as a kinase domain. To study LeEIX2 mode of action, it is essential to identify the potential interactors involved after EIX perception. Here, we describe the in vivo co-IP methodology used for protein interaction verification and ethylene and ROS (reactive oxygen species) measurements used for physiological effects assessment.

Key words

LRR-RLP Ethylene-inducing xylanase MAMP Defense responses Co-IP ROS 

Notes

Acknowledgments

This work was partly supported by the Israel Science Foundation administered by the Israel Academy of Science and Humanities no. 388/12 and Research Grant Award no. IS-4842-15R from BARD, the United States–Israel Binational Agriculture Research and Development Fund and by the United States-Israel Binational Science Foundation no. 2013227.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Meirav Leibman-Markus
    • 1
  • Silvia Schuster
    • 1
  • Adi Avni
    • 1
    Email author
  1. 1.Department of Molecular Biology and Ecology of PlantsTel-Aviv UniversityTel-AvivIsrael

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