Analysis of PAMP-Triggered ROS Burst in Plant Immunity
The plant perception of pathogen-associated molecular patterns triggers a plethora of cellular immune responses. One of these responses is a rapid and transient burst of reactive oxygen species (ROS) mediated by plasma membrane-localized NADPH oxidases. The ROS burst requires a functional receptor complex and the contribution of several additional regulatory components. In laboratory conditions, the ROS burst can be detected a few minutes after the treatment with an immunogenic microbial elicitor. For these reasons, the elicitor-triggered ROS burst has been often exploited as readout to probe the contribution of plant components to early immune responses. Here, we describe a detailed protocol for the measurement of elicitor-triggered ROS burst in a simple, fast, and easy manner.
Key wordsReactive oxygen species ROS flg22 PAMP Immunity
We thank Rosa Lozano-Durán for critical reading and suggestions on this manuscript. We also thank members of the Macho laboratory, past and present members of Cyril Zipfel’s group (The Sainsbury Laboratory, UK), and several other groups for their contribution to the optimization of this protocol over the years. Research in the Macho laboratory is supported by the Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences), and the Chinese 1000 Talents Program.
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