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Analysis of PAMP-Triggered ROS Burst in Plant Immunity

  • Yuying Sang
  • Alberto P. MachoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1578)

Abstract

The plant perception of pathogen-associated molecular patterns triggers a plethora of cellular immune responses. One of these responses is a rapid and transient burst of reactive oxygen species (ROS) mediated by plasma membrane-localized NADPH oxidases. The ROS burst requires a functional receptor complex and the contribution of several additional regulatory components. In laboratory conditions, the ROS burst can be detected a few minutes after the treatment with an immunogenic microbial elicitor. For these reasons, the elicitor-triggered ROS burst has been often exploited as readout to probe the contribution of plant components to early immune responses. Here, we describe a detailed protocol for the measurement of elicitor-triggered ROS burst in a simple, fast, and easy manner.

Key words

Reactive oxygen species ROS flg22 PAMP Immunity 

Notes

Acknowledgments

We thank Rosa Lozano-Durán for critical reading and suggestions on this manuscript. We also thank members of the Macho laboratory, past and present members of Cyril Zipfel’s group (The Sainsbury Laboratory, UK), and several other groups for their contribution to the optimization of this protocol over the years. Research in the Macho laboratory is supported by the Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences), and the Chinese 1000 Talents Program.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.CAS Center for Excellence in Molecular Plant Sciences, Shanghai Center for Plant Stress BiologyShanghai Institutes of Biological Sciences, Chinese Academy of SciencesShanghaiChina

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