Abstract
Genedata Biologics is a novel informatics platform specifically designed for biologics R&D. Here, we discuss the main principles employed in designing such a platform, focusing on antibody engineering. To illustrate, we present a case study of how the platform effectively supports an antibody optimization workflow and ensures the successful integration and analysis of all relevant sequence, expression, assay, and analytics data.
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Schlinkmann KM, Hillenbrand M, Rittner A, Künz M, Strohner R, Plückthun A (2012) Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J Mol Biol 422(3):414–428. doi:10.1016/j.jmb.2012.05.039 Epub 2012 Jun 6. PubMed PMID: 22683350
Dokarry M, Laurendon C, O'Maille PE (2012) Automating gene library synthesis by structure-based combinatorial protein engineering: examples from plant sesquiterpene synthases. Methods Enzymol 515:21–42. doi:10.1016/B978-0-12-394290-6.00002-1 PubMed PMID: 22999168
Debaene F, Wagner-Rousset E, Colas O, Ayoub D, Corvaïa N, Van Dorsselaer A, Beck A, Cianférani S (2013) Time resolved native ion-mobility mass spectrometry to monitor dynamics of IgG4 Fab arm exchange and "bispecific" monoclonal antibody formation. Anal Chem 85(20):9785–9792. doi:10.1021/ac402237v Epub 2013 Sep 26 . PubMed PMID: 24007193
Birdsall RE, Shion H, Kotch FW, Xu A, Porter TJ, Chen W (2015) A rapid on-line method for mass spectrometric confirmation of a cysteine-conjugated antibody-drug-conjugate structure using multidimensional chromatography. MAbs 7(6):1036–1044. doi:10.1080/19420862.2015.1083665 Epub 2015 Aug 25. PubMed PMID: 26305867; PubMed Central PMCID: PMC4966495
Zambrano R, Jamroz M, Szczasiuk A, Pujols J, Kmiecik S, Ventura S (2015) AGGRESCAN3D (A3D): server for prediction of aggregation properties of protein structures. Nucleic Acids Res 43(W1):W306–W313. doi:10.1093/nar/gkv359 Epub 2015 Apr 16. PubMed PMID: 25883144; PubMed Central PMCID: PMC4489226.
Chaparro-Riggers JF, Polizzi KM, Bommarius AS (2007) Better library design: data-driven protein engineering. Biotechnol J 2(2):180–191 Review PubMed PMID: 17183506
Rajpal A, Beyaz N, Haber L, Cappuccilli G, Yee H, Bhatt RR, Takeuchi T, Lerner RA, Crea R (2005) A general method for greatly improving the affinity of antibodies by using combinatorial libraries. Proc Natl Acad Sci U S A 102(24):8466–8471 Epub 2005 Jun 6. PubMed PMID: 15939870; PubMed Central PMCID: PMC1143585
Kempeni J (1999) Preliminary results of early clinical trials with the fully human anti-TNFalpha monoclonal antibody D2E7. Ann Rheum Dis 58(Suppl 1):I70–I72 Review. PubMed PMID: 10577977; PubMed Central PMCID: PMC1766582
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Wendt, M., Cappuccilli, G. (2017). Data-Driven Antibody Engineering Using Genedata Biologics™. In: Tiller, T. (eds) Synthetic Antibodies. Methods in Molecular Biology, vol 1575. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6857-2_15
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DOI: https://doi.org/10.1007/978-1-4939-6857-2_15
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