Abstract
1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene. The movement of ACC across the plasma membrane (PM) has been implicated in various physiological contexts during environmental adaptation and differentiation in higher plants. A PM-localized transporter in Arabidopsis thaliana, LYSINE HISTIDINE TRANSPORTER1 (LHT1) participates in the uptake of ACC, implicating a class of amino-acid transporters in the transport of ACC. Here, we describe the method for assaying uptake of ACC into the plant cells using an Arabidopsis mesophyll protoplast system.
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Acknowledgment
This work was supported by grant from National Research Foundation of Korea (NRF) funded by the Ministry of Science, (2015R1C1A2A01052950 for S. L. 2015R1D1A1A01058039 for M.-S.S).
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Song, WY., Lee, S., Soh, MS. (2017). Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System. In: Binder, B., Eric Schaller, G. (eds) Ethylene Signaling. Methods in Molecular Biology, vol 1573. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6854-1_4
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DOI: https://doi.org/10.1007/978-1-4939-6854-1_4
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