Abstract
Analytical detection of the plant hormone ethylene is an important prerequisite in physiological studies. Real-time and super sensitive detection of trace amounts of ethylene gas is possible using laser-based photo-acoustic spectroscopy. This Chapter will provide some background on the technique, compare it with conventional gas chromatography, and provide a detailed user-friendly hand-out on how to operate the machine and the software. In addition, this Chapter provides some tips and tricks for designing and performing physiological experiments suited for ethylene detection with laser-based photo-acoustic spectroscopy.
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Acknowledgments
BVdP is a postdoctoral fellow of the Research Foundation Flanders (FWO Vlaanderen). This work was supported by projects from Ghent University (Bijzonder Onderzoeksfonds, 01B02112) and the Research Foundation Flanders (FWO Vlaanderen, G.0656.13N) to DVDS.
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Van de Poel, B., Van Der Straeten, D. (2017). Plant Ethylene Detection Using Laser-Based Photo-Acoustic Spectroscopy. In: Binder, B., Eric Schaller, G. (eds) Ethylene Signaling. Methods in Molecular Biology, vol 1573. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6854-1_2
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DOI: https://doi.org/10.1007/978-1-4939-6854-1_2
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