Abstract
Gold nanorod (GNR) based label-free sensing has been attractive due to its unique property of localized surface plasmon resonance (LSPR). Compared to bulk gold, the SPR of GNRs is more sensitive to the refractive index change caused by biological binding in the close proximity. Numerous studies have reported biological detection in solution based GNR probes. However, the biosensing has the intrinsic problems of fluctuating readings and short storage time due to nanoparticle aggregation. In contrast, a chip-based nanorod biosensor is a more robust and reliable platform. We have developed a nanoplasmonic biosensor in a chip format by immobilizing functionalized GNRs on a (3-mercaptopropyl)trimethoxysilane modified glass substrate. The covalent Au-S bond ensures a strong GNR deposition on the substrate. This biochip exhibits a high sensitivity and stability when exposed to physiological buffer with high ionic strength. Another advantage of GNR as optical transducer is its LSPR peak dependence on the aspect ratio, which provides an ideal multiplexed detection mechanism. GNRs of different sizes that exhibit distinct SPR peaks are combined and deposited on designated spots of a glass substrate. The spectral shift of the respective peaks upon the biological binding are monitored for simultaneous detection of specific analytes. Coupled with a microplate reader, this spatially resolved GNR array biochip results in a high-throughput assay of samples as well as multiplexed detection in each sample. Since most biological molecules such as antibodies and DNA can be linked to GNR using previously reported surface chemistry protocol, the label-free nanosensor demonstrated here is an effective tool for protein/DNA array analysis, especially for detection of disease biomarkers.
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References
Giri S, Trewyn BG, Lin VS (2007) Mesoporous silica nanomaterial-based biotechnological and biomedical delivery systems. Nanomedicine (Lond) 2(1):99–111
Lee SH, Sung JH, Park TH (2012) Nanomaterial-based biosensor as an emerging tool for biomedical applications. Ann Biomed Eng 40(6):1384–1397
Oyelere AK et al (2007) Peptide-conjugated gold nanorods for nuclear targeting. Bioconjug Chem 18(5):1490–1497
Chylek P (1986) Absorption and scattering of light by small particles. Appl Opt 25(18):3166
Bennett HS, Rosasco GJ (1978) Resonances in the efficiency factors for absorption: Mie scattering theory. Appl Optics 17(4):491–493
Upadhyayula VK (2012) Functionalized gold nanoparticle supported sensory mechanisms applied in detection of chemical and biological threat agents: a review. Anal Chim Acta 715:1–18
Yu L, Andriola A (2010) Quantitative gold nanoparticle analysis methods: a review. Talanta 82(3):869–875
Dreaden EC et al (2012) The golden age: gold nanoparticles for biomedicine. Chem Soc Rev 41(7):2740–2779
Anker JN et al (2008) Biosensing with plasmonic nanosensors. Nat Mater 7(6):442–453
Jain PK et al (2006) Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. J Phys Chem B 110(14):7238–7248
Chou SF et al (2004) Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance. Biosens Bioelectron 19(9):999–1005
Sim HR, Wark AW, Lee HJ (2010) Attomolar detection of protein biomarkers using biofunctionalized gold nanorods with surface plasmon resonance. Analyst 135(10):2528–2532
Ye X et al (2013) Using binary surfactant mixtures to simultaneously improve the dimensional tunability and monodispersity in the seeded growth of gold nanorods. Nano Lett 13(2):765–771
Gulati A, Liao H, Hafner JH (2006) Monitoring gold nanorod synthesis by localized surface plasmon resonance. J Phys Chem B 110(45):22323–22327
Wang Y, Tang L (2015) Multiplexed gold nanorod array biochip for multi-sample analysis. Biosens Bioelectron 67:18–24
Wang X et al (2015) Gold nanorod biochip functionalization by antibody thiolation. Talanta 136:1–8
Wang Y, Tang L (2013) Chemisorption assembly of Au nanorods on mercaptosilanized glass substrate for label-free nanoplasmon biochip. Anal Chim Acta 796:122–129
Orendorff CJ, Murphy CJ (2006) Quantitation of metal content in the silver-assisted growth of gold nanorods. J Phys Chem B 110(9):3990–3994
Lim DK et al (2011) Highly uniform and reproducible surface-enhanced Raman scattering from DNA-tailorable nanoparticles with 1-nm interior gap. Nat Nanotechnol 6(7):452–460
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Mei, Z., Wang, Y., Tang, L. (2017). Gold Nanorod Array Biochip for Label-Free, Multiplexed Biological Detection. In: Rasooly, A., Prickril, B. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1571. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6848-0_9
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DOI: https://doi.org/10.1007/978-1-4939-6848-0_9
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