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Histamine Receptors as Drug Targets pp 183–208Cite as

Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst

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Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Polymorphonuclear leukocytes (neutrophils) form the first line of defence in humans. After activation, they migrate through vessel walls toward the invading agent. The intruding pathogens are eliminated through the action of released proteases and by production of reactive oxygen species (ROS). Histamine, a biogenic amine with various physiological and pathological effects, is known to modulate the immune response of neutrophils, e.g., it blunts the production of ROS in neutrophils after binding to the histamine-2 receptor. To detect the ROS in neutrophils, various methods are used. Among these, luminophore-enhanced chemiluminescence is a very sensitive and easy tool for studying the effects of various compounds on ROS production in neutrophils. On the other hand, its selectivity is questionable. However, employing cell-free systems producing selective ROS types, the inhibitory effects of tested compounds against distinct ROS may be evaluated. In this chapter, we describe the methods for the evaluation of ROS modulating effects of histamine agonists/antagonists in human neutrophils by using chemiluminescence methods.

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Acknowledgments

The study was supported by the MEYS of the Czech Republic (LD14030), Slovak Research and Development Agency (APVV-0052-10), and the Scientific Grant Agency of the Slovak Republic (VEGA 2/0029/16). OV and TP were supported by the project no. LQ1605 from the National Program of Sustainability II (MEYS CR) and by the project FNUSA-ICRC no. CZ.1.05/1.1.00/02.0123 (OP VaVpI).

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Authors and Affiliations

  1. Institute of Biophysics, Czech Academy of Sciences, Královopolská, 135, 612 65, Brno, Czech Republic

    Ondřej Vašíček, Tomáš Perečko & Milan Číž

  2. International Clinical Research Center, St. Anne’s University Hospital, Brno, Czech Republic

    Ondřej Vašíček & Tomáš Perečko

  3. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia

    Viera Jančinová, Silvia Pažoureková & Radomír Nosáľ

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  1. Ondřej Vašíček
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  2. Tomáš Perečko
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  3. Viera Jančinová
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  4. Silvia Pažoureková
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  5. Radomír Nosáľ
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  6. Milan Číž
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Correspondence to Milan Číž .

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Editors and Affiliations

  1. Department of Pharmacology Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Ekaterini Tiligada

  2. Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast, Northern Ireland, United Kingdom

    Madeleine Ennis

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Vašíček, O., Perečko, T., Jančinová, V., Pažoureková, S., Nosáľ, R., Číž, M. (2017). Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst. In: Tiligada, E., Ennis, M. (eds) Histamine Receptors as Drug Targets. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6843-5_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6843-5_7

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